目的 研究黄芩提取物(SGE)对脂多糖(LPS)诱导BV2细胞炎症反应的作用,并探讨其可能的作用机制。方法 四甲基偶氮唑蓝(MTT)法检测细胞存活率;Griess试剂检测细胞上清中NO水平;ELISA法检测细胞上清中白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α水平;Western Blot检测BV2细胞中TLR4蛋白的表达。结果 与正常对照组比较,LPS诱导BV2细胞上清中NO、IL-1β、IL-6、TNF-α水平显著增高(P<0.01);与LPS模型组比较,SGE 100 μg·mL-1显著降低LPS诱导的BV2细胞上清中炎症因子NO (P<0.01)、IL-1β (P<0.01)、IL-6 (P<0.01)、TNF-α (P<0.05)水平。深入研究发现,SGE可显著降低LPS诱导BV2细胞中TLR4蛋白的表达。结论 SGE能够显著抑制LPS所诱导的BV2细胞炎症反应,机制可能与抑制TLR4炎症通路相关。
Abstract
OBJECTIVE To investigate the anti-inflammatory effects of extract of Scutellaria baicalensis Georgi (SGE) and underlying mechanism by using LPS-induced microglial BV2 cells. METHODS MTT assay was used to observe the cell viability. The content of NO in cell supernatant was measured by Griess reagent. The levels of IL-1β, IL-6 and TNF-α were detected by ELISA kits. The intracellular TLR4 expression was assayed by Western blotting. RESULTS The levels of NO, IL-1β, IL-6 and TNF-α were significantly increased induced by LPS in the supernatant of BV2 cells (all P<0.01). However, co-treatment with SGE 100 μg·mL-1 significantly decreased the production of related inflammatory factors including NO (P<0.01), IL-1β(P<0.01), IL-6 (P<0.01) and TNF-α (P<0.05). Furthermore, SGE significantly inhibited the TLR4 expression induced by LPS in BV2 cells. CONCLUSION SGE is able to alleviate LPS-induced inflammatory responses in BV2 cells through down-regulation of TLR4 protein expression suggesting that SGE has therapeutic potential for the treatment of neuroinflammatory diseases.
关键词
黄芩提取物 /
脂多糖 /
BV2细胞 /
神经炎症
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Key words
Scutellaria baicalensis Georgi extract /
lipopolysaccharide /
BV2 cells /
neuroinflammation
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中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金面上项目资助(81473383,81573645);中国医学科学院医学与健康科技创新工程资助(2016-I2M-3-007); 国家科技重大专项重大新药创制资助(2017ZX09101003-003-019);北京协和医学院研究生创新基金(2017-1007-02)
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